For rapid and efficient extraction of plasmid DNA from bacterial cells. The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer.
Fast extraction time
High yield
Standard version or endotoxin-free version (<0.10 EU/µg) available
Specifications
Method:
Anion-Exchange
Sample size:
200 - 800 ml high-copy plasmid / 500 - 1600 ml low-copy plasmid