For rapid and efficient extraction of plasmid DNA from bacterial cells. The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.
Fast extraction time
High yield
Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator
Specifications
I-Blue Mini Plasmid Kit // I-Blue Midi Plasmid Kit
Method:
Spin column // Anion-Exchange
Sample size:
1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmid