High specification, economically priced real-time thermocycler. The Eco 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5 µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ± 0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Eco 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.
Features:
Validated volume per well: 5 to 20 µl
High temperature uniformity of ± 0.1°C across the whole block instantly after every temperature change
40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
Genotype in 4 minutes with over 99% accuracy
Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
Dual LED excitation (452 nm to 486 nm and 542 nm to 582 nm)
4 emission filters (505 nm to 545 nm, 562 nm to 596 nm, 604 nm to 644 nm, 665 nm to 705 nm)
Data is always collected in all four filters for all wells
Distinguishes 5000 and 10000 template copies with 99 percent confidence
